A Baumannii | Real Time Polymerase Chain Reaction | Polymerase Chain Reaction

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PrimerDesign TMLtd Quantification of Acinetobacter baumannii hypothetical protein sequence gene For general laboratory and research use only Advanced kit 150 tests Quantification of Acinetobacter baumannii genomes. genesig Advanced kit handbook HB10.03.05 1 Introduction to Acinetobacter baumannii Acinetobacter baumannii is a Gram-negative bacterium of the Moraxellaceae family. The genome of this bacterium is around 4Mbp in length, arranged in a single, circular chromosome. The bacterium us
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  Acinetobacter baumanniiQuantification of  For general laboratory and research use only  hypothetical protein sequence gene PrimerDesign Ltd TM 150 testsAdvanced kit 1Quantification of Acinetobacter baumannii genomes.genesig Advanced kit handbook HB10.03.05  Acinetobacter baumannii is a Gram-negative bacterium of the Moraxellaceae family. Thegenome of this bacterium is around 4Mbp in length, arranged in a single, circular chromosome. The bacterium usually resides in soil or water but increasingly is found innosocomial infections where antibiotic resistance is increasing. Porins in the outer cellmembrane of the bacterium are greatly reduced in size compared to other Gram-negativespecies and contribute to increased antibiotic resistance.A. baumannii has been found to be the cause of up to 10% of Gram-negative bacterialinfections in Europe and the US. Individuals with weakened immune systems are particularly susceptible to infection by this species. Due to the ability of the bacterium to proliferate onmany surfaces and environments including dry and static surfaces, nosocomial infections may occur by patient contact with hospital equipment, especially invasive apparatus where thebacterium can easily be introduced into the blood stream.A. baumanni infections are known to be involved in respiratory and urinary tract infections aswell as causing pneumonia, septicaemia and meningitis. Introduction to Acinetobacter baumannii 2Quantification of Acinetobacter baumannii genomes.genesig Advanced kit handbook HB10.03.05  Specificity  The primers and probe sequences in this kit have 100% homology with a broad range of clinically relevant reference sequences based on a comprehensive bioinformatics analysis.ThePrimerDesign™genesigKitforAcinetobacterbaumannii(A.baumannii)GenomesisdesignedfortheinvitroquantificationofA.baumanniigenomes.Thekitisdesignedtohave the broadest detection profile possible whilst remaining specific to the A. baumannii genome.Theprimersandprobesequencesinthiskithave100%homologywithabroadrangeoclinically relevant reference sequences based on a comprehensive bioinformatics analysis.Ifyourequirefurtherinformation,orhaveaspecificquestionaboutthedetectionprofileof  thiskitthenpleasesendane.mailtoenquiries@primerdesign.co.ukandourbioinformatics team will answer your question.3Quantification of Acinetobacter baumannii genomes.genesig Advanced kit handbook HB10.03.05  Kit Contents ã A. baumannii specific primer/probe mix 150 reactions BROWN)   FAM labeled, BHQ quenched ã A. baumannii positive control template for Standard curve RED)ã Internal extraction control DNA 150 reactions BLUE)ã Internal extraction control primer/probe mix 150 reactions BROWN)   Choice of VIC channel or CY5 channel ã Endogenous ACTB primer/probe mix 150 reactions BROWN)   FAM labeled, BHQ quenched ã RNAse/DNAse free water Reagents and equipment to be supplied by the user  Real-Time PCR InstrumentDNA extraction kit ThiskitisdesignedtoworkwellwithallprocessesthatyieldhighqualityDNAwithminimalPCR inhibitors. oasig TM  Lyophilised 2 x qPCR Mastermix ThiskitisdesignedtoworkwellwithallcommerciallyavailableMastermixes.However,werecommend the use of oasig TM  Lyophilsed 2x qPCR MasterMix. Pipettors and TipsVortex and centrifugeThin walled 1.5 ml PCR reaction tubes 4Quantification of Acinetobacter baumannii genomes.genesig Advanced kit handbook HB10.03.05
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